SLIT2 antibody (AA 934-1529)

Catalog No. ABIN7602678

This Rabbit Polyclonal antibody specifically detects SLIT2 in WB, ELISA and FACS. It exhibits reactivity toward Human.

Quick Overview for SLIT2 antibody (AA 934-1529) (ABIN7602678)

Target: SLIT2 (Slit Homolog 2 (Drosophila) (SLIT2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLIT2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-SLIT2 Antibody

Binding Specificity: AA 934-1529

Purpose: Anti-SLIT2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SLIT2 Antibody Picoband® (ABIN7602678). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SLIT2 recombinant protein (Position: N934-S1529).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bagri, A., Marin, O., Plump, A. S., Mak, J., Pleasure, S. J., Rubenstein, J. L. R., Tessier-Lavigne, M. Slit proteins prevent midline crossing and determine the dorsoventral position of major axonal pathways in the mammalian forebrain. Neuron 33: 233-248, 2002. 2. Brose, K., Bland, K. S., Wang, K. H., Arnott, D., Henzel, W., Goodman, C. S., Tessier-Lavigne, M., Kidd, T. Slit proteins bind Robo receptors and have an evolutionarily conserved role in repulsive axon guidance. Cell 96: 795-806, 1999. 3. Georgas, K., Burridge, L., Smith, K., Holmes, G. P., Chenevix-Trench, G., Ioannou, P. A., Little, M. H. Assignment of the human slit homologue SLIT2 to human chromosome band 4p15.2. Cytogenet. Cell Genet. 86: 246-247, 1999.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for SLIT2

Target: SLIT2 (Slit Homolog 2 (Drosophila) (SLIT2))

Alternative Name: SLIT2

Background:

Synonyms: Tryptophan 5-hydroxylase 1, Tryptophan 5-monooxygenase 1, TPH1, TPH, TPRH, TRPH

Tissue Specificity: Isoform 2 seems to be less widely expressed than isoform 1.

Background: Slit homolog 2 protein is a protein that in humans is encoded by the SLIT2 gene. This gene encodes a member of the slit family of secreted glycoproteins, which are ligands for the Robo family of immunoglobulin receptors. Slit proteins play highly conserved roles in axon guidance and neuronal migration and may also have functions during other cell migration processes including leukocyte migration. Members of the slit family are characterized by an N-terminal signal peptide, four leucine-rich repeats, nine epidermal growth factor repeats, and a C-terminal cysteine knot. Proteolytic processing of this protein gives rise to an N-terminal fragment that contains the four leucine-rich repeats and five epidermal growth factor repeats and a C-terminal fragment that contains four epidermal growth factor repeats and the cysteine knot. Both full length and cleaved proteins are secreted extracellularly and can function in axon repulsion as well as other specific processes. Alternative splicing results in multiple transcript variants.

Molecular Weight: 170 kDa

Gene ID: 9353

UniProt: O94813

Pathways: Regulation of Actin Filament Polymerization, Regulation of Cell Size, Smooth Muscle Cell Migration