IFT52 antibody (AA 95-410)

Catalog No. ABIN7602687

This Rabbit Polyclonal antibody specifically detects IFT52 in ELISA, WB, IF, IHC and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for IFT52 antibody (AA 95-410) (ABIN7602687)

Target: IFT52 (Intraflagellar Transport 52 Homolog (IFT52))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IFT52 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-IFT52 Antibody

Binding Specificity: AA 95-410

Purpose: Anti-IFT52 Antibody Picoband®

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Anti-IFT52 Antibody Picoband® (ABIN7602687). Tested in ELISA, IF, IHC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human LIS1/PAFAH1B1 recombinant protein (Position: I95-R410). Human PAFAH1B1 shares 100% amino acid (aa) sequence identity with both mouse and rat PAFAH1B1.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Human, Mouse, Rat
Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Chen, X., Wang, X., Jiang, C., Xu, M., Liu, Y., Qi, R., Qi, X., Sun, X., Xie, P., Liu, Q., Yan, B., Sheng, X., Zhao, C. IFT52 as a novel candidate for ciliopathies involving retinal degeneration. Invest. Ophthal. Vis. Sci. 59: 4581-4589, 2018. 2. Dupont, M. A., Humbert, C., Huber, C., Siour, Q., Guerrera, I. C., Jung, V., Christensen, A., Pouliet, A., Garfa-Traore, M., Nitschke, P., Injeyan, M., Millar, K., and 11 others. Human IFT52 mutations uncover a novel role for the protein in microtubule dynamics and centrosome cohesion. Hum. Molec. Genet. 28: 2720-2737, 2019. 3. Girisha, K. M., Shukla, A., Trujillano, D., Bhavani, G. S., Hebbar, M., Kadavigere, R., Rolfs, A. A homozygous nonsense variant in IFT52 is associated with a human skeletal ciliopathy. Clin. Genet. 90: 536-539, 2016.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for IFT52

Target: IFT52 (Intraflagellar Transport 52 Homolog (IFT52))

Alternative Name: IFT52

Background:

Synonyms: 70 kDa ribosomal protein S6 kinase 1 antibody, KS6B1_HUMAN antibody, p70 alpha antibody, P70 beta 1 antibody, p70 ribosomal S6 kinase alpha antibody, p70 ribosomal S6 kinase beta 1 antibody, p70 S6 kinase alpha antibody, P70 S6 Kinase antibody, p70 S6 kinase alpha 1 antibody, p70 S6 kinase alpha 2 antibody, p70 S6K antibody, p70 S6K-alpha antibody, p70 S6KA antibody, p70(S6K) alpha antibody, p70(S6K)-alpha antibody, p70-alpha antibody, p70-S6K 1 antibody, p70-S6K antibody, P70S6K antibody, P70S6K1 antibody, p70S6Kb antibody, PS6K antibody, Ribosomal protein S6 kinase 70 kDa polypeptide 1 antibody, Ribosomal protein S6 kinase beta 1 antibody, Ribosomal protein S6 kinase beta-1 antibody, Ribosomal protein S6 kinase I antibody, RPS6KB1 antibody, S6K antibody, S6K-beta-1 antibody, S6K1 antibody, Serine/threonine kinase 14 alpha antibody, Serine/threonine-protein kinase 14A antibody, STK14A antibody

Tissue Specificity: Expressed in all tissues.

Background: This gene encodes a conserved proline-rich protein that is a component of the intraflagellar transport-B (IFT-B) core complex. The encoded protein is essential for the integrity of the IFT-B core complex, and for biosynthesis and maintenance of cilia. Mutations in this gene are associated with ciliopathy that affects the skeleton.

Molecular Weight: 45 kDa

Gene ID: 51098

UniProt: Q9Y366