Clathrin Heavy Chain (CLTC) (AA 967-1668) antibody

Catalog No. ABIN7602704

This anti- antibody is a Rabbit Polyclonal antibody detecting in WB, IF, ELISA, IHC, FACS and ICC. Suitable for Human, Mouse and Rat.

Quick Overview for Clathrin Heavy Chain (CLTC) (AA 967-1668) antibody (ABIN7602704)

Target: Clathrin Heavy Chain (CLTC)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details

Binding Specificity: AA 967-1668

Purpose: Anti-Clathrin heavy chain/CLTC Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Clathrin heavy chain/CLTC Antibody Picoband® (ABIN7602704). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Clathrin heavy chain/CLTC recombinant protein (Position: R967-Q1668).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/mL, -
1. Argani, P., Lui, M. Y., Couturier, J., Bouvier, R., Fournet, J.-C., Ladanyi, M. A novel CLTC-TFE3 gene fusion in pediatric renal adenocarcinoma with t(X,17)(p11.2,q23). Oncogene 22: 5374-5378, 2003. 2. Cools, J., Wlodarska, I., Somers, R., Mentens, N., Pedeutour, F., Maes, B., De Wolf-Peeters, C., Pauwels, P., Hagemeijer, A., Marynen, P. Identification of novel fusion partners of ALK, the anaplastic lymphoma kinase, in anaplastic large-cell lymphoma and inflammatory myofibroblastic tumor. Genes Chromosomes Cancer 34: 354-362, 2002. 3. Deborde, S., Perret, E., Gravotta, D., Deora, A., Salvarezza, S., Schreiner, R., Rodriguez-Boulan, E. Clathrin is a key regulator of basolateral polarity. Nature 452: 719-723, 2008.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details

Target: Clathrin Heavy Chain (CLTC)

Alternative Name: CLTC

Background:

Synonyms: Tumor necrosis factor receptor superfamily member 18, Glucocorticoid-induced TNFR-related protein, CD357, Tnfrsf18, Gitr

Tissue Specificity: Detected in thymus and in activated splenocytes.

Background: Clathrin heavy chain 1 is a protein that in humans is encoded by the CLTC gene. Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains.

Molecular Weight: 192 kDa

Gene ID: 1213

UniProt: Q00610