CES1 antibody (AA 99-206)

Catalog No. ABIN7602719

This anti-CES1 antibody is a Mouse Monoclonal antibody detecting CES1 in WB, IHC, ICC, IF and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for CES1 antibody (AA 99-206) (ABIN7602719)

Target: CES1 (Carboxylesterase 1 (CES1))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CES1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: 3F10

Product Details anti-CES1 Antibody

Binding Specificity: AA 99-206

Purpose: Anti-Liver Carboxylesterase 1/CES1 Antibody Picoband® (monoclonal, 3F10)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Liver Carboxylesterase 1/CES1 Antibody Picoband® (monoclonal, 3F10) (ABIN7602719). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human Liver Carboxylesterase 1/CES1 recombinant protein (Position: E99-A206).

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. "Entrez Gene: CES1 carboxylesterase 1 (monocyte/macrophage serine esterase 1)". 2. Markey GM (Feb 2011). "Carboxylesterase 1 (Ces1): from monocyte marker to major player". J Clin Pathol. 64 (2): 107-9. 3. Zhu HJ, Appel DI, Johnson JA, Chavin KD, Markowitz JS (Jan 2009). "Role of carboxylesterase 1 and impact of natural genetic variants on the hydrolysis of trandolapril". Biochem Pharmacol. 77 (7): 1266-72.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CES1

Target: CES1 (Carboxylesterase 1 (CES1))

Alternative Name: CES1

Background:

Synonyms: Fatty acid-binding protein, heart, Fatty acid-binding protein 3, Heart-type fatty acid-binding protein, H-FABP, Fabp3

Tissue Specificity: Heart, but also skeletal muscle, kidney, brain and mammary gland.

Background: Liver carboxylesterase 1 also known as carboxylesterase 1 (CES1, hCE-1 or CES1A1) is an enzyme that in humans is encoded by the CES1 gene. This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. This enzyme is the major liver enzyme and functions in liver drug clearance. Mutations of this gene cause carboxylesterase 1 deficiency. Three transcript variants encoding three different isoforms have been found for this gene.

Molecular Weight: 63 kDa

Gene ID: 1066

UniProt: P23141

Pathways: Monocarboxylic Acid Catabolic Process