WWC1 antibody (AA 990-1080)

Catalog No. ABIN7602724

The Rabbit Polyclonal anti-WWC1 antibody has been validated for WB, ELISA, ICC, IF and FACS. It is suitable to detect WWC1 in samples from Human, Mouse and Rat.

Quick Overview for WWC1 antibody (AA 990-1080) (ABIN7602724)

Target: WWC1 (WW and C2 Domain Containing 1 (WWC1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WWC1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-WWC1 Antibody

Binding Specificity: AA 990-1080

Purpose: Anti-KIBRA/WWC1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-KIBRA/WWC1 Antibody Picoband® (ABIN7602724). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human KIBRA/WWC1 recombinant protein (Position: D990-Q1080).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Buther, K., Plaas, C., Barnekow, A., Kremerskothen, J. KIBRA is a novel substrate for protein kinase C-zeta. Biochem. Biophys. Res. Commun. 317: 703-707, 2004. 2. Duning, K., Wennmann, D. O., Bokemeyer, A., Reissner, C., Wershching, H., Thomas, C., Buschert, J., Guske, K., Franzke, V., Floel, A., Lohmann, H., Knecht, S., and 13 others. Common exonic missense variants in the C2 domain of the human KIBRA protein modify lipid binding and cognitive performance. Transl. Psychiat. 3: e272, 2013. Note: Electronic Article. 3. Kremerskothen, J., Plaas, C., Buther, K., Finger, I., Veltel, S., Matanis, T., Liedtke, T., Barnekow, A. Characterization of KIBRA, a novel WW domain-containing protein. Biochem. Biophys. Res. Commun. 300: 862-867, 2003.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for WWC1

Target: WWC1 (WW and C2 Domain Containing 1 (WWC1))

Alternative Name: WWC1

Background:

Synonyms: Elongator complex protein 3, Help3, ELP3

Tissue Specificity: Expressed at low levels in the more differentiated suprabasal regions of the small intestine, and at higher levels in the colon, mainly in the upper region and in scattered cells throughout the remaining epithelium. Also expressed in epithelial cells of bladder, ileum and stomach and at lower levels in pancreas and earskin. The phosphorylated form is nearly exclusively expressed in goblet cells of the small intestine and in the lumen-proximal cells of the colon (at protein level). Also expressed in jejunum and duodenum.

Background: Protein KIBRA also known as kidney and brain expressed protein (KIBRA) or WW domain-containing protein 1 (WWC1) is a protein that in humansl is encoded by the WWC1 gene. The protein encoded by this gene is a cytoplasmic phosphoprotein that interacts with PRKC-zeta and dynein light chain-1. Alleles of this gene have been found that enhance memory in some individuals. Three transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 130 kDa

Gene ID: 23286