IKZF1 antibody (C-Term)

Catalog No. ABIN7602788

This anti-IKZF1 antibody is a Mouse Monoclonal antibody detecting IKZF1 in WB. Suitable for Human.

Quick Overview for IKZF1 antibody (C-Term) (ABIN7602788)

Target: IKZF1 (IKAROS Family Zinc Finger 1 (Ikaros) (IKZF1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IKZF1 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 5F12H7

Product Details anti-IKZF1 Antibody

Binding Specificity: C-Term

Purpose: Anti-Ikaros/IKZF1 Antibody Picoband® (monoclonal, 5F12H7)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Ikaros/IKZF1 Antibody Picoband® (monoclonal, 5F12H7) (ABIN7602788). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Ikaros, different from the related mouse sequence by five amino acids.

Isotype: IgG1

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
1. Georgopoulos K, Moore DD, Derfler B (December 1992). "Ikaros, an early lymphoid-specific transcription factor and a putative mediator for T cell commitment". Science 258 (5083): 808-12. 2. Hahm K, Ernst P, Lo K, Kim GS, Turck C, Smale ST (November 1994). "The lymphoid transcription factor LyF-1 is encoded by specific, alternatively spliced mRNAs derived from the Ikaros gene". Mol Cell Biol 14 (11): 7111-23.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for IKZF1

Target: IKZF1 (IKAROS Family Zinc Finger 1 (Ikaros) (IKZF1))

Alternative Name: IKZF1

Background:

Synonyms: Serum paraoxonase/arylesterase 1, PON 1

Tissue Specificity: Plasma, liver, kidney, heart, brain, small intestine and lung. In the plasma, associated with HDL.

Background: DNA-binding protein Ikaros is a protein that in humans is encoded by the IKZF1 gene. This gene encodes a transcription factor that belongs to the family of zinc-finger DNA-binding proteins associated with chromatin remodeling. The expression of this protein is restricted to the fetal and adult hemo-lymphopoietic system, and it functions as a regulator of lymphocyte differentiation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. Most isoforms share a common C-terminal domain, which contains two zinc finger motifs that are required for hetero- or homo-dimerization, and for interactions with other proteins. The isoforms, however, differ in the number of N-terminal zinc finger motifs that bind DNA and in nuclear localization signal presence, resulting in members with and without DNA-binding properties. Only a few isoforms contain the requisite three or more N-terminal zinc motifs that confer high affinity binding to a specific core DNA sequence element in the promoters of target genes. The non-DNA-binding isoforms are largely found in the cytoplasm, and are thought to function as dominant-negative factors.

Molecular Weight: 55-65 kDa

Gene ID: 10320

UniProt: Q13422

Pathways: Production of Molecular Mediator of Immune Response