CD16 antibody (C-Term)

Catalog No. ABIN7602814

The Rabbit Polyclonal anti-CD16 antibody has been validated for WB. It is suitable to detect CD16 in samples from Mouse.

Quick Overview for CD16 antibody (C-Term) (ABIN7602814)

Target: CD16

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD16 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CD16 Antibody

Binding Specificity: C-Term

Purpose: Anti-CD16/Fcgr3 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-CD16/Fcgr3 Antibody Picoband® (ABIN7602814). Tested in WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of mouse CD16/Fcgr3, which shares 73.7% amino acid (aa) sequence identity with rat CD16/Fcgr3.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse
1. Pinheiro da Silva, F., Aloulou, M., Skurnik, D., Benhamou, M., Andremont, A., Velasco, I. T., Chiamolera, M., Verbeek, J. S., Launay, P., Monteiro, R. C. CD16 promotes Escherichia coli sepsis through an FcR-gamma inhibitory pathway that prevents phagocytosis and facilitates inflammation. Nature Med. 13: 1368-1374, 2007. 2. Robinson, J. I., Carr, I. M., Cooper, D. L., Rashid, L. H., Martin, S. G., Emery, P., Isaacs, J. D., Barton, A., BRAGGSS, Wilson, A. G., Barrett, J. H., Morgan, A. W. Confirmation of association of FCGR3B but not FCGR3A copy number with susceptibility to autoantibody positive rheumatoid arthritis. Hum. Mutat. 33: 741-749, 2012.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CD16

Target: CD16

Alternative Name: Fcgr3

Background:

Synonyms: Low affinity immunoglobulin gamma Fc region receptor III-A, CD16a antigen, Fc-gamma RIII-alpha, Fc-gamma RIII, Fc-gamma RIIIa, FcRIII, FcRIIIa, FcR-10, IgG Fc receptor III-2, CD16a, FCGR3A, CD16A, FCG3, FCGR3, IGFR3

Tissue Specificity: Expressed on natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placental trophoblasts.

Background: Low affinity immunoglobulin gamma Fc region receptor III-A is a protein that in humans is encoded by the FCGR3A gene. This gene encodes a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other other antibody-dependent responses. This gene (FCGR3A) is highly similar to another nearby gene (FCGR3B) located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, whereas FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 70 kDa

Gene ID: 14131

UniProt: P08508

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process