COX8A antibody (C-Term)

Catalog No. ABIN7602820

The Rabbit Polyclonal anti-COX8A antibody has been validated for WB, IHC and FACS. It is suitable to detect COX8A in samples from Mouse and Rat.

Quick Overview for COX8A antibody (C-Term) (ABIN7602820)

Target: COX8A (Cytochrome C Oxidase Subunit VIIIA (Ubiquitous) (COX8A))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COX8A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-COX8A Antibody

Binding Specificity: C-Term

Purpose: Anti-COX8A Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-COX8A Antibody (ABIN7602820). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of mouse COX8A, which shares 100% and 66.7% amino acid (aa) sequence identity with human and rat COX8A, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for COX8A

Target: COX8A (Cytochrome C Oxidase Subunit VIIIA (Ubiquitous) (COX8A))

Alternative Name: Cox8a

Background:

Synonyms: Mediator of RNA polymerase II transcription subunit 9, Mediator complex subunit 9, MED9, MED25,

Tissue Specificity: Expressed in fetal brain, fetal lung, fetal liver, heart, brain, placenta, lung, liver, muscle, kidney and pancreas.

Background: The protein encoded by this gene is the terminal enzyme of the respiratory chain, coupling the transfer of electrons from cytochrome c to molecular oxygen, with the concomitant production of a proton electrochemical gradient across the inner mitochondrial membrane. In addition to 3 mitochondrially encoded subunits, which perform the catalytic function, the eukaryotic enzyme contains nuclear-encoded smaller subunits, ranging in number from 4 in some organisms to 10 in mammals. It has been proposed that nuclear-encoded subunits may be involved in the modulation of the catalytic function. This gene encodes one of the nuclear-encoded subunits.

Molecular Weight: 8-10 kDa

Gene ID: 12868

UniProt: Q64445