CMBL antibody (C-Term)

Catalog No. ABIN7602830

Product Details anti-CMBL Antibody

Target: CMBL (Carboxymethylenebutenolidase Homolog (CMBL))

Binding Specificity: C-Term

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CMBL antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Purpose: Anti-LOC134147/CMBL Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-L/CMBL Antibody Picoband® (ABIN7602830). Tested in Flow Cytometry, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of mouse LOC134147/CMBL, which shares 88.2% and 94.1% amino acid (aa) sequence identity with human and rat LOC134147/CMBL, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse
1. Ishizuka, T., Fujimori, I., Kato, M., Noji-Sakikawa, C., Saito, M., Yoshigae, Y., Kubota, K., Kurihara, A., Izumi, T., Ikeda, T., Okazaki, O. Human carboxymethylenebutenolidase as a bioactivating hydrolase of olmesartan medoxomil in liver and intestine. J. Biol. Chem. 285: 11892-11902, 2010.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CMBL

Target: CMBL (Carboxymethylenebutenolidase Homolog (CMBL))

Alternative Name: Cmbl (CMBL Products)

Background:

Synonyms: Meiotic recombination protein REC8 homolog, Cohesin Rec8p, REC8, REC8L1

Tissue Specificity: Expressed in testis and thymus. Expressed in the B-cell lines WI-L2-NS and Namalwa.

Background: CMBL is a cysteine hydrolase of the dienelactone hydrolase family that is highly expressed in liver cytosol. CMBL preferentially cleaves cyclic esters, and it activates medoxomil-ester prodrugs in which the medoxomil moiety is linked to an oxygen atom.

Molecular Weight: 28 kDa

Gene ID: 69574

UniProt: Q8R1G2