RAG1 antibody (C-Term)

Catalog No. ABIN7602835

The Rabbit Polyclonal anti-RAG1 antibody has been validated for WB. It is suitable to detect RAG1 in samples from Mouse and Human.

Quick Overview for RAG1 antibody (C-Term) (ABIN7602835)

Target: RAG1 (Recombination Activating Gene 1 (RAG1))

Reactivity: Mouse, Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAG1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RAG1 Antibody

Binding Specificity: C-Term

Purpose: Anti-RAG1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-RAG1 Antibody Picoband® (ABIN7602835). Tested in WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human RAG1, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse
1. Agrawal, A., Eastman, Q. M., Schatz, D. G. Transposition mediated by RAG1 and RAG2 and its implications for the evolution of the immune system. Nature 394: 744-751, 1998. 2. Blanquet, V., Turleau, C., Goossens, M., Besmond, C. Assignment of the human recombination activating gene 1 (RAG1) to the 14q21.3-q22.2 region. Genomics 13: 488-489, 1992. 3. Corneo, B., Moshous, D., Gungor, T., Wulffraat, N., Philippet, P., Le Deist, F., Fischer, A., de Villartay, J.-P. Identical mutations in RAG1 or RAG2 genes leading to defective V(D)J recombinase activity can cause either T-B-severe combined immune deficiency or Omenn syndrome. Blood 97: 2772-2776, 2001.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for RAG1

Target: RAG1 (Recombination Activating Gene 1 (RAG1))

Alternative Name: RAG1

Background:

Synonyms: Interferon alpha/beta receptor 1,IFN-R-1,IFN-alpha/beta receptor 1,Cytokine receptor class-II member 1,Cytokine receptor family 2 member 1,CRF2-1,Type I interferon receptor 1,IFNAR1,IFNAR,

Tissue Specificity: IFN receptors are present in all tissues and even on the surface of most IFN-resistant cells. Isoform 1, isoform 2 and isoform 3 are expressed in the IFN-alpha sensitive myeloma cell line U266B1. Isoform 2 and isoform 3 are expressed in the IFN-alpha resistant myeloma cell line U266R. Isoform 1 is not expressed in IFN-alpha resistant myeloma cell line U266R. .

Background: Recombination activating gene 1 also known as RAG-1 is a protein that in humans is encoded by the RAG1 gene. The protein encoded by this gene is involved in activation of immunoglobulin V-D-J recombination. The encoded protein is involved in recognition of the DNA substrate, but stable binding and cleavage activity also requires RAG2. Defects in this gene can be the cause of several diseases.

Molecular Weight: 120 kDa

Gene ID: 5896

UniProt: P15918