ARL1 antibody (C-Term)

Catalog No. ABIN7602848

This anti-ARL1 antibody is a Rabbit Polyclonal antibody detecting ARL1 in WB, IHC, IF and FACS. Suitable for Human.

Quick Overview for ARL1 antibody (C-Term) (ABIN7602848)

Target: ARL1 (ADP Ribosylation Factor Like GTPase 1 (ARL1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-ARL1 Antibody

Binding Specificity: C-Term

Purpose: Anti-ARL1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-ARL1 Antibody Picoband® (ABIN7602848). Tested in Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human ARL1, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL/mL, Human
Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/mL/1x10^6 cells, Human
1. Gross, M. B. Personal Communication. Baltimore, Md. 7/25/2012. 2. Hong, J.-X., Lee, F.-J. S., Patton, W. A., Lin, C. Y., Moss, J., Vaughan, M. Phospholipid- and GTP-dependent activation of cholera toxin and phospholipase D by human ADP-ribosylation factor-like protein 1 (HARL1). J. Biol. Chem. 273: 15872-15876, 1998. 3. Lu, L., Tai, G., Hong, W. Autoantigen golgin-97, an effector of Arl1 GTPase, participates in traffic from the endosome to the trans-Golgi network. Molec. Biol. Cell 15: 4426-4443, 2004.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for ARL1

Target: ARL1 (ADP Ribosylation Factor Like GTPase 1 (ARL1))

Alternative Name: ARL1

Background:

Synonyms: ATP-dependent RNA helicase DDX1, DEAD box protein 1, DEAD box protein retinoblastoma, DBP-RB, DDX1

Tissue Specificity: Highest levels of transcription in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin including the retina, brain, and spinal cord.

Background: ADP-ribosylation factor-like protein 1 is a protein that in humans is encoded by the ARL1 gene. The protein encoded by this gene belongs to the ARL (ADP-ribosylation factor-like) family of proteins, which are structurally related to ADP-ribosylation factors (ARFs). ARFs, described as activators of cholera toxin (CT) ADP-ribosyltransferase activity, regulate intracellular vesicular membrane trafficking, and stimulate a phospholipase D (PLD) isoform. Although, ARL proteins were initially thought not to activate CT or PLD, later work showed that they are weak stimulators of PLD and CT in a phospholipid dependent manner. Alternative splicing results in multiple transcript variants encoding different isoforms.

Molecular Weight: 20 kDa

Gene ID: 400

UniProt: P40616