DAP antibody (C-Term)

Catalog No. ABIN7602875

This anti-DAP antibody is a Rabbit Polyclonal antibody detecting DAP in IHC, WB, IF, ICC and FACS. Suitable for Human.

Quick Overview for DAP antibody (C-Term) (ABIN7602875)

Target: DAP (Death-Associated Protein (DAP))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DAP antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-DAP Antibody

Binding Specificity: C-Term

Purpose: Anti-DAP1/DAP Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-DAP1/DAP Antibody Picoband® (ABIN7602875). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human DAP1/DAP, which shares 92.9% amino acid (aa) sequence identity with both mouse and rat DAP1/DAP.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Deiss, L. P., Feinstein, E., Berissi, H., Cohen, O., Kimchi, A. Identification of a novel serine/threonine kinase and a novel 15-kD protein as potential mediators of the gamma interferon-induced cell death. Genes Dev. 9: 15-30, 1995. 2. Feinstein, E., Druck, T., Kastury, K., Berissi, H., Goodart, S. A., Overhauser, J., Kimchi, A., Huebner, K. Assignment of DAP1 and DAPK: genes that positively mediate programmed cell death triggered by IFN-gamma--to chromosome regions 5p12.2 and 9q34.1, respectively. Genomics 29: 305-307, 1995.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for DAP

Target: DAP (Death-Associated Protein (DAP))

Alternative Name: DAP

Background:

Synonyms: Platelet basic protein, PBP, C-X-C motif chemokine 7, Leukocyte-derived growth factor, LDGF, Macrophage-derived growth factor, MDGF, Small-inducible cytokine B7, PPBP, CTAP3, CXCL7, SCYB7, TGB1, THBGB1, NAP-2

Background: Death-associated protein 1 is a protein that in humans is encoded by the DAP gene. This gene encodes a basic, proline-rich, 15-kD protein. The protein acts as a positive mediator of programmed cell death that is induced by interferon-gamma. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Molecular Weight: 15 kDa

Gene ID: 1611

UniProt: P51397

Pathways: Positive Regulation of Endopeptidase Activity, Feeding Behaviour