MGEA5 antibody (C-Term)

Catalog No. ABIN7602902

This anti-MGEA5 antibody is a Rabbit Polyclonal antibody detecting MGEA5 in WB, IHC, ICC, IF and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for MGEA5 antibody (C-Term) (ABIN7602902)

Target: MGEA5 (Meningioma Expressed Antigen 5 (Hyaluronidase) (MGEA5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MGEA5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-MGEA5 Antibody

Binding Specificity: C-Term

Purpose: Anti-MGEA5/OGA Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-MGEA5/OGA Antibody (ABIN7602902). Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at C-terminus of human OGA, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for MGEA5

Target: MGEA5 (Meningioma Expressed Antigen 5 (Hyaluronidase) (MGEA5))

Alternative Name: OGA

Background:

Synonyms: Protein Bop, BH3-only protein, Retrotransposon Gag-like protein 10, RTL10, BOP, C22orf29

Tissue Specificity: Ubiquitously expressed.

Background: Protein O-GlcNAcase (EC 3.2.1.169, OGA, glycoside hydrolase O-GlcNAcase, O-GlcNAcase, BtGH84, O-GlcNAc hydrolase) is an enzyme with systematic name (protein)-3-O- (N-acetyl-D-glucosaminyl)-L-serine/threonine N-acetylglucosaminyl hydrolase. The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications.

Molecular Weight: 130 kDa

Gene ID: 10724

UniProt: O60502

Pathways: Positive Regulation of Peptide Hormone Secretion, Regulation of Carbohydrate Metabolic Process