TRAM1 antibody (C-Term)

Catalog No. ABIN7602938

This anti-TRAM1 antibody is a Rabbit Polyclonal antibody detecting TRAM1 in WB and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for TRAM1 antibody (C-Term) (ABIN7602938)

Target: TRAM1 (Translocation Associated Membrane Protein 1 (TRAM1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRAM1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-TRAM1 Antibody

Binding Specificity: C-Term

Purpose: Anti-TRAM1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-TRAM1 Antibody Picoband® (ABIN7602938). Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human TRAM1, which shares 89.5% amino acid (aa) sequence identity with mouse and rat TRAM1.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Gorlich, D., Hartmann, E., Prehn, S., Rapoport, T. A. A protein of the endoplasmic reticulum involved early in polypeptide translocation. Nature 357: 47-52, 1992.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for TRAM1

Target: TRAM1 (Translocation Associated Membrane Protein 1 (TRAM1))

Alternative Name: TRAM1

Background:

Synonyms: Olfactomedin-4, OLM4, Antiapoptotic protein GW112, G-CSF-stimulated clone 1 protein, hGC-1, hOLfD, OLFM4, GW112, UNQ362, PRO698

Tissue Specificity: Expressed during myeloid lineage development. Much higher expression in bone marrow neutrophils than in peripheral blood neutrophils (at protein level). Strongly expressed in the prostate, small intestine and colon and moderately expressed in the bone marrow and stomach. Overexpressed in some pancreatic cancer tissues.

Background: TRAM1(Translocation-Associating Membrane Protein 1), By crosslinking and reconstitution of canine proteoliposomes, followed by microsequencing and PCR screening of canine kidney and HeLa cell cDNA libraries, Gorlich et al.(1992) isolated cDNAs encoding TRAM(translocating chain-associating membrane protein). The International Radiation Hybrid Mapping Consortium mapped the TRAM gene to chromosome 8. Sequence analysis predicted that human TRAM is a 374-amino acid, 8-pass transmembrane protein that shares 95 % amino acid identity with the canine protein. Functional analysis indicated that TRAM influences glycosylation and is stimulatory or required for the translocation of secretory proteins.

Molecular Weight: 55-60 kDa

Gene ID: 23471

UniProt: Q15629