GAPDH antibody (C-Term) (HRP)

Catalog No. ABIN7602946

The Rabbit Polyclonal anti-GAPDH antibody has been validated for WB. It is suitable to detect GAPDH in samples from Human, Mouse, Rat, Chicken, Monkey and Zebrafish (Danio rerio).

Quick Overview for GAPDH antibody (C-Term) (HRP) (ABIN7602946)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivity: Human, Mouse, Rat, Chicken, Monkey, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GAPDH antibody is conjugated to HRP

Application: Western Blotting (WB)

Product Details anti-GAPDH Antibody (HRP)

Binding Specificity: C-Term

Purpose: Anti-GAPDH Antibody Picoband® (HRP)

Cross-Reactivity: Chicken, Human, Monkey, Mouse, Rat, Zebrafish (Danio rerio)

Cross-Reactivity (Details): HRP-GAPDH antibody is human, mouse, rat, rabbit, and chicken reactive.

Characteristics: Anti-GAPDH Antibody (HRP) (-HRP). Tested in WB applications. This antibody reacts with Human, Mouse, Rat, Monkey, Chicken, Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: HRP-GAPDH antibody is affinity chromatography purified via peptide column.

Immunogen: HRP-GAPDH antibody was raised against a synthetic peptide containing 16 amino acids near the carboxy terminus of GAPDH.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.02-0.1 μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 0.5 mg/mL

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: HRP-GAPDH antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH

Background:

Synonyms: HRP-GAPDH, Glyceraldehyde-3-phosphate dehydrogenase, G3PDH, GAPD

Tissue Specificity: Widely expressed, with the highest expression in lung, spleen and thymus.

Background: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. It also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export (1). GAPDH also plays a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer's and Huntington's disease (2). GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.

Sequence Similarities: Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A4 subfamily.

Molecular Weight: 36 kDa

UniProt: P04406