GABARAP antibody (C-Term)

Catalog No. ABIN7602949

The Rabbit Monoclonal anti-GABARAP antibody has been validated for WB, IHC, IF, ICC and FACS. It is suitable to detect GABARAP in samples from Human, Mouse and Rat.

Quick Overview for GABARAP antibody (C-Term) (ABIN7602949)

Target: GABARAP (GABA(A) Receptor-Associated Protein (GABARAP))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This GABARAP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: ACHE-7

Product Details anti-GABARAP Antibody

Binding Specificity: C-Term

Purpose: Anti-GABARAP Monoclonal Antibody

Characteristics: Anti-GABARAP Monoclonal Antibody (ABIN7602949). Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human GABARAP GABARAP is cleaved at its carboxyl terminus, which leads to conjugation by either of the phospholipids phosphatidylethanolamine or phosphatidylserine. This processing converts GABARAP from a type I to a type II membrane bound form involved in autophagosome biogenesis. Processing of GABARAP involves cleavage by Atg4 family members followed by conjugation by the E1 and E2 like enzymes Atg7 and Atg3.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000
IHC 1:100-1:500
ICC/IF 1:50-1:200
FC 1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Concentration: Lot specific

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for GABARAP

Target: GABARAP (GABA(A) Receptor-Associated Protein (GABARAP))

Alternative Name: GABARAP

Background:

Synonyms: Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial,1.2.4.1,PDHE1-A type I,PDHA1,PHE1A,

Tissue Specificity: Ubiquitous.

Molecular Weight: 18 kDa

UniProt: O95166

Pathways: Autophagy