Leucine Rich Transmembrane and 0-Methyltransferase Domain Containing (LRTOMT) (Middle Region) antibody

Catalog No. ABIN7602986

This anti- antibody is a Rabbit Polyclonal antibody detecting in WB and IHC. Suitable for Human, Mouse and Rat.

Quick Overview for Leucine Rich Transmembrane and 0-Methyltransferase Domain Containing (LRTOMT) (Middle Region) antibody (ABIN7602986)

Target: Leucine Rich Transmembrane and 0-Methyltransferase Domain Containing (LRTOMT)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details

Binding Specificity: Middle Region

Purpose: Anti-LRTOMT Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-LRTOMT Antibody (ABIN7602986). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human LRTOMT.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.5-1 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 1-2 μg/mL, Human, Mouse, Rat
1. Charif M, Bounaceur S, Abidi O, Nahili H, Rouba H, Kandil M, Boulouiz R, Barakat A (December 2012). "The c.242G>A mutation in LRTOMT gene is responsible for a high prevalence of deafness in the Moroccan population". Molecular Biology Reports. 39 (12): 11011-6. 2. "Entrez Gene: Leucine rich transmembrane and O-methyltransferase domain containing" 3. Riahi Z, Bonnet C, Zainine R, Louha M, Bouyacoub Y, Laroussi N, Chargui M, Kefi R, Jonard L, Dorboz I, Hardelin JP, Salah SB, Levilliers J, Weil D, McElreavey K, Boespflug OT, Besbes G, Abdelhak S, Petit C (2014). "Whole exome sequencing identifies new causative mutations in Tunisian families with non-syndromic deafness". PLoS One. 9 (6): e99797.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details

Target: Leucine Rich Transmembrane and 0-Methyltransferase Domain Containing (LRTOMT)

Alternative Name: LRTOMT

Background:

Synonyms: Transmembrane O-methyltransferase, Catechol O-methyltransferase 2, Protein LRTOMT2, LRTOMT, COMT2

Background: Leucine rich transmembrane and O-methyltransferase domain containing is a protein that in humans is encoded by the LRTOMT gene. It is mapped to 11q13.4. This gene has evolved in primates as a fusion of two ancestral neighboring genes, Lrrc51 and Tomt, which exist as two independent genes in rodents. The fusion gene contains some shared exons, but encodes structurally unrelated proteins, LRTOMT1 and LRTOMT2. Those variants that utilize the more centromere-proximal 3' terminal exon (short transcript form) encode LRTOMT1, while those variants that use a more centromere-distal 3' terminal exon (long transcript form) encode the LRTOMT2 protein. There is a small region within one of the exons of this gene that contains overlapping alternate reading frames for both LRTOMT1 and LRTOMT2. LRTOMT1 shares similarity with the protein encoded by mouse Lrrc51, while LRTOMT2 shares similarity with the protein encoded by mouse Tomt. Alternative splicing results in multiple transcript variants, encoding different isoforms of both LRTOMT1 and LRTOMT2. The LRTOMT1 protein is a leucine-rich repeat-containing protein, while the LRTOMT2 protein is a catechol-O-methyltransferase that catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to a hydroxyl group of catechols and is essential for auditory and vestibular function. Mutations in this gene have been associated with nonsyndromic deafness.

Molecular Weight: 28 kDa

Gene ID: 220074

Pathways: Sensory Perception of Sound