Cyclin T1 antibody (Middle Region)

Catalog No. ABIN7602991

The Mouse Monoclonal anti-Cyclin T1 antibody has been validated for WB and FACS. It is suitable to detect Cyclin T1 in samples from Human and Monkey.

Quick Overview for Cyclin T1 antibody (Middle Region) (ABIN7602991)

Target: Cyclin T1 (CCNT1)

Reactivity: Human, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cyclin T1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Clone: 3B7

Product Details anti-Cyclin T1 Antibody

Binding Specificity: Middle Region

Purpose: Anti-Cyclin T1 Antibody Picoband® (monoclonal, 3B7)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Cyclin T1 Antibody Picoband® (monoclonal, 3B7) (ABIN7602991). Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Cyclin T1, different from the related mouse sequence by one amino acid.

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Monkey
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. "Entrez Gene: CCNT1 cyclin T1". 2. Peng J, Zhu Y, Milton JT, Price DH (April 1998). "Identification of multiple cyclin subunits of human P-TEFb". Genes Dev. 12 (5): 755-62. 3. Wei P, Garber ME, Fang SM, Fischer WH, Jones KA (March 1998). "A novel CDK9-associated C-type cyclin interacts ly with HIV-1 Tat and mediates its high-affinity, loop-specific binding to TAR RNA". Cell92 (4): 451-62.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for Cyclin T1

Target: Cyclin T1 (CCNT1)

Alternative Name: CCNT1

Background:

Synonyms: Histone-binding protein RBBP4, Chromatin assembly factor 1 subunit C, CAF-1 subunit C, Chromatin assembly factor I p48 subunit, CAF-I 48 kDa subunit, CAF-I p48, Nucleosome-remodeling factor subunit RBAP48, Retinoblastoma-binding protein 4, RBBP-4, Retinoblastoma-binding protein p48, RBBP4, RBAP48

Background: Cyclin-T1 is a protein that in humans is encoded by the CCNT1 gene. This gene encodes a member of the highly conserved cyclin C subfamily. The encoded protein tightly associates with cyclin-dependent kinase 9, and is a major subunit of positive transcription elongation factor b (p-TEFb). In humans, there are multiple forms of positive transcription elongation factor b, which may include one of several different cyclins along with cyclin-dependent kinase 9. The complex containing the encoded cyclin and cyclin-dependent kinase 9 acts as a cofactor of human immunodeficiency virus type 1 (HIV-1) Tat protein, and is both necessary and sufficient for full activation of viral transcription. This cyclin and its kinase partner are also involved in triggering transcript elongation through phosphorylation of the carboxy-terminal domain of the largest RNA polymerase II subunit. Overexpression of this gene is implicated in tumor growth. Alternative splicing results in multiple transcript variants.

Molecular Weight: 81 kDa

Gene ID: 904

UniProt: O60563