Fibrinogen beta Chain antibody (Middle Region)

Catalog No. ABIN7602994

The Mouse Monoclonal anti-Fibrinogen beta Chain antibody has been validated for WB and IHC. It is suitable to detect Fibrinogen beta Chain in samples from Human.

Quick Overview for Fibrinogen beta Chain antibody (Middle Region) (ABIN7602994)

Target: Fibrinogen beta Chain (FGB)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Fibrinogen beta Chain antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: 6D12

Product Details anti-Fibrinogen beta Chain Antibody

Binding Specificity: Middle Region

Purpose: Anti-Fibrinogen beta chain/FGB Antibody Picoband® (monoclonal, 6D12)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Fibrinogen beta chain/FGB Antibody Picoband® (monoclonal, 6D12) (ABIN7602994). Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human FGB, different from the related mouse sequence by three amino acids, and from the related rat sequence by five amino acids.

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
1. "Entrez Gene: FGB fibrinogen beta chain". 2. Chung, D. W., Que, B. G., Rixon, M. W., Mace, M., Jr., Davie, E. W. Characterization of complementary deoxyribonucleic acid and genomic deoxyribonucleic acid for the beta chain of human fibrinogen. Biochemistry 22: 3244-3250, 1983. 3. Spena, S., Duga, S., Asselta, R., Malcovati, M., Peyvandi, F., Tenchini, M. L. Congenital afibrinogenemia: first identification of splicing mutations in the fibrinogen B-beta-chain gene causing activation of cryptic splice sites. Blood 100: 4478-4484, 2002.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Fibrinogen beta Chain

Target: Fibrinogen beta Chain (FGB)

Alternative Name: FGB

Background:

Synonyms: Bis (5'-adenosyl)-triphosphatase, AP3A hydrolase, AP3Aase, Diadenosine 5',5'''-P1,P3-triphosphate hydrolase, Dinucleosidetriphosphatase, Fragile histidine triad protein, FHIT

Tissue Specificity: Low levels expressed in all tissues tested. Phospho-FHIT observed in liver and kidney, but not in brain and lung. Phospho-FHIT undetected in all tested human tumor cell lines.

Background: Fibrinogen beta chain, mapped to 4q31.3, is also known asFGB. The protein encoded by this gene is the beta component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in this gene lead to several disorders, including afibrinogenemia, dysfibrinogenemia, hypodysfibrinogenemia and thrombotic tendency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 56 kDa

Gene ID: 2244

UniProt: P02675