CLTB antibody (Middle Region)

Catalog No. ABIN7603036

The Rabbit Polyclonal anti-CLTB antibody has been validated for WB, IHC and FACS. It is suitable to detect CLTB in samples from Human, Mouse and Rat.

Quick Overview for CLTB antibody (Middle Region) (ABIN7603036)

Target: CLTB (Clathrin, Light Chain B (CLTB))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CLTB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-CLTB Antibody

Binding Specificity: Middle Region

Purpose: Anti-CLTB Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-CLTB Antibody Picoband® (ABIN7603036). Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human CLTB, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Brodsky, F. M., Galloway, C. J., Blank, G. S., Jackson, A. P., Seow, H.-F., Drickamer, K., Parham, P. Localization of clathrin light-chain sequences mediating heavy-chain binding and coated vesicle diversity. Nature 326: 203-205, 1987. 2. Gross, M. B. Personal Communication. Baltimore, Md. 4/20/2011. 3. Jackson, A. P., Parham, P. Structure of human clathrin light chains: conservation of light chain polymorphism in three mammalian species. J. Biol. Chem. 263: 16688-16695, 1988.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for CLTB

Target: CLTB (Clathrin, Light Chain B (CLTB))

Alternative Name: CLTB

Background:

Synonyms: CLTB, Clathrin light chain B, Lcb

Background: Clathrin, light chain B is a protein in humans that is encoded by the CLTB gene. Clathrin is a large, soluble protein composed of heavy and light chains. It functions as the main structural component of the lattice-type cytoplasmic face of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. This gene encodes one of two clathrin light chain proteins which are believed to function as regulatory elements. Alternative splicing results in multiple transcript variants.

Molecular Weight: 32 kDa

Gene ID: 1212

UniProt: P09497

Pathways: Synaptic Membrane