COX6A2 antibody (Middle Region)

Catalog No. ABIN7603039

The Rabbit Polyclonal anti-COX6A2 antibody has been validated for WB. It is suitable to detect COX6A2 in samples from Mouse and Rat.

Quick Overview for COX6A2 antibody (Middle Region) (ABIN7603039)

Target: COX6A2 (Cytochrome C Oxidase Subunit VIa Polypeptide 2 (COX6A2))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COX6A2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-COX6A2 Antibody

Binding Specificity: Middle Region

Purpose: Anti-Cox6a2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Cox6a2 Antibody Picoband® (ABIN7603039). Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of mouse Cox6a2, which shares 66.7% and 100% amino acid (aa) sequence identity with human and rat Cox6a2, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
1. Bachman, N. J., Riggs, P. K., Siddiqui, N., Makris, G. J., Womack, J. E., Lomax, M. I. Structure of the human gene (COX6A2) for the heart/muscle isoform of cytochrome c oxidase subunit VIa and its chromosomal location in humans, mice, and cattle. Genomics 42: 146-151, 1997. 2. Inoue, M., Uchino, S., Iida, A., Noguchi, S., Hayashi, S., Takahashi, T., Fujii, K., Komaki, H., Takeshita, E., Nonaka, I., Okada, Y., Yoshizawa, T., Van Lommel, L., Schuit, F., Goto, Y., Mimaki, M., Nishino, I. COX6A2 variants cause a muscle-specific cytochrome c oxidase deficiency. Ann. Neurol. 86: 193-202, 2019. 3. Quintens, R., Singh, S., Lemaire, K., De Bock, K., Granvik, M., Schraenen, A., Vroegrijk, I. O. C. M., Costa, V., Van Noten, P., Lambrechts, D., Lehnert, S., Van Lommel, L., and 10 others. Mice deficient in the respiratory chain gene Cox6a2 are protected against high-fat diet-induced obesity and insulin resistance. PLoS One 8: e56719, 2013. Note: Electronic Article.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for COX6A2

Target: COX6A2 (Cytochrome C Oxidase Subunit VIa Polypeptide 2 (COX6A2))

Alternative Name: Cox6a2

Background:

Synonyms: Kelch repeat and BTB domain-containing protein 2, BTB and kelch domain-containing protein 1, KBTBD2, BKLHD1, KIAA1489

Tissue Specificity: Detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon.

Background: Cytochrome c oxidase subunit VIa polypeptide 2 is a protein that in humans is encoded by the COX6A2 gene. Cytochrome c oxidase (COX), the terminal enzyme of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. It is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may be involved in the regulation and assembly of the complex. This nuclear gene encodes polypeptide 2 (heart/muscle isoform) of subunit VIa, and polypeptide 2 is present only in striated muscles. Polypeptide 1 (liver isoform) of subunit VIa is encoded by a different gene, and is found in all non-muscle tissues. These two polypeptides share 66 % amino acid sequence identity.

Molecular Weight: 11 kDa

Gene ID: 12862

UniProt: P43023