G3BP1 antibody (Middle Region)

Catalog No. ABIN7603051

The Rabbit Polyclonal anti-G3BP1 antibody has been validated for WB, IHC, IF, ICC and FACS. It is suitable to detect G3BP1 in samples from Human.

Quick Overview for G3BP1 antibody (Middle Region) (ABIN7603051)

Target: G3BP1 (GTPase Activating Protein (SH3 Domain) Binding Protein 1 (G3BP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This G3BP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-G3BP1 Antibody

Binding Specificity: Middle Region

Purpose: Anti-G3BP/G3BP1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-G3BP/G3BP1 Antibody Picoband® (ABIN7603051). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human G3BP/G3BP1, which shares 64.7% amino acid (aa) sequence identity with mouse G3BP1.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Costa, M., Ochem, A., Staub, A., Falaschi, A. Human DNA helicase VIII: a DNA and RNA helicase corresponding to the G3BP protein, an element of the Ras transduction pathway. Nucleic Acids Res. 27: 817-821, 1999. 2. Parker, F., Maurier, F., Delumeau, I., Duchesne, M., Faucher, D., Debussche, L., Dugue, A., Schweighoffer, F., Tocque, B. A Ras-GTPase-activating protein SH3-domain-binding protein. Molec. Cell. Biol. 16: 2561-2569, 1996. 3. Tourriere, H., Chebli, K., Zekri, L., Courselaud, B., Blanchard, J. M., Bertrand, E., Tazi, J. The RasGAP-associated endoribonuclease G3BP assembles stress granules. J. Cell Biol. 160: 823-831, 2003.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for G3BP1

Target: G3BP1 (GTPase Activating Protein (SH3 Domain) Binding Protein 1 (G3BP1))

Alternative Name: G3BP1

Background:

Synonyms: Estradiol 17-beta-dehydrogenase 1, 17-beta-hydroxysteroid dehydrogenase type 1, 17-beta-HSD 1, 20 alpha-hydroxysteroid dehydrogenase, 20-alpha-HSD, E2DH, Placental 17-beta-hydroxysteroid dehydrogenase, Short chain dehydrogenase/reductase family 28C member 1, HSD17B1, E17KSR, EDH17B1, EDH17B2, EDHB17, SDR28C1

Tissue Specificity: Almost ubiquitous. Largely expressed in adult heart, skeletal muscle, ovary, and fetal kidney. Moderate amounts in adult liver, kidney, pancreas, spleen, and fetal lung, and small amounts in placenta, adult lung, prostate, testis, colon, fetal brain and liver.

Background: Ras GTPase-activating protein-binding protein 1 is an enzyme that in humans is encoded by the G3BP1 gene. This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

Molecular Weight: 68 kDa

Gene ID: 10146

UniProt: Q13283

Pathways: SARS-CoV-2 Protein Interactome