SFPQ antibody (Middle Region)

Catalog No. ABIN7603089

This Rabbit Polyclonal antibody specifically detects SFPQ in WB, IHC, IF, ICC and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for SFPQ antibody (Middle Region) (ABIN7603089)

Target: SFPQ (Splicing Factor Proline/glutamine-Ric (SFPQ))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SFPQ antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-SFPQ Antibody

Binding Specificity: Middle Region

Purpose: Anti-SFPQ Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SFPQ Antibody Picoband® (ABIN7603089). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human SFPQ, identical to the related mouse sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human, Mouse, Rat
1. Clark, J., Lu, Y.-J., Sidhar, S. K., Parker, C., Gill, S., Smedley, D., Hamoudi, R., Linehan, W. M., Shipley, J., Cooper, C. S. Fusion of splicing factor genes PSF and NonO (p54-nrb) to the TFE3 gene in papillary renal cell carcinoma. Oncogene 15: 2233-2239, 1997. 2. Duong, H. A., Robles, M. S., Knutti, D., Weitz, C. J. A molecular mechanism for circadian clock negative feedback. Science 332: 1436-1439, 2011. 3. Patton, J. G., Porro, E. B., Galceran, J., Tempst, P., Nadal-Ginard, B. Cloning and characterization of PSF, a novel pre-mRNA splicing factor. Genes Dev. 7: 393-406, 1993

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for SFPQ

Target: SFPQ (Splicing Factor Proline/glutamine-Ric (SFPQ))

Alternative Name: SFPQ

Background:

Synonyms: UDP-glucuronosyltransferase 1-6, UDPGT 1-6, UGT1*6, UGT1-06, UGT1.6, A1, P-nitrophenol-specific UDPGT, UDP-glucuronosyltransferase 1A6, UGT1A6, Ugt1a6, Ugt1

Background: Splicing factor, proline- and glutamine-rich is a protein that in humans is encoded by the SFPQ gene. Enables DNA binding activity, histone deacetylase binding activity, and protein homodimerization activity. Involved in several processes, including alternative mRNA splicing, via spliceosome, positive regulation of oxidative stress-induced intrinsic apoptotic signaling pathway, and regulation of transcription by RNA polymerase II. Acts upstream of or within double-strand break repair via homologous recombination. Located in chromatin, nuclear matrix, and paraspeckles.

Molecular Weight: 100 kDa

Gene ID: 6421

UniProt: P23246