SH3GL3 antibody (Middle Region)

Catalog No. ABIN7603091

This anti-SH3GL3 antibody is a Rabbit Polyclonal antibody detecting SH3GL3 in WB and FACS. Suitable for Human, Rat and Mouse.

Quick Overview for SH3GL3 antibody (Middle Region) (ABIN7603091)

Target: SH3GL3 (Endophilin-A3 (SH3GL3))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SH3GL3 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-SH3GL3 Antibody

Binding Specificity: Middle Region

Purpose: Anti-SH3GL3 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SH3GL3 Antibody Picoband® (ABIN7603091). Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human SH3GL3, which shares 95.8% amino acid (aa) sequence identity with mouse and rat SH3GL3.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Giachino, C., Lantelme, E., Lanzetti, L., Saccone, S., Della Valle, G., Migone, N. A novel SH3-containing human gene family preferentially expressed in the central nervous system. Genomics 41: 427-434, 1997. 2. Li, S., Qiao, Y., Di, Q., Le, X., Zhang, L., Zhang, X., Zhang, C., Cheng, J., Zong, S., Koide, S. S., Miao, S., Wang, L. Interaction of SH3P13 and DYDC1 protein: a germ cell component that regulates acrosome biogenesis during spermiogenesis. Europ. J. Cell Biol. 88: 509-520, 2009. Note: Erratum: Europ. J. Cell Biol. 98: 51-52, 2019. 3. Petrelli, A., Gilestro, G. F., Lanzardo, S., Comoglio, P. M., Migone, N., Giordano, S. The endophilin-CIN85-Cbl complex mediates ligand-dependent downregulation of c-Met. Nature 416: 187-190, 2002.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for SH3GL3

Target: SH3GL3 (Endophilin-A3 (SH3GL3))

Alternative Name: SH3GL3

Background:

Synonyms: Interleukin-36 alpha, FIL1 epsilon, Interleukin-1 epsilon, IL-1 epsilon, Interleukin-1 family member 6, IL-1F6, IL36A, FIL1E, IL1E, IL1F6

Tissue Specificity: Expressed in immune system and fetal brain, but not in other tissues tested or in multiple hematopoietic cell lines. Predominantly expressed in skin keratinocytes but not in fibroblasts, endothelial cells or melanocytes. Increased in lesional psoriasis skin.

Background: Endophilin-A3 is a protein that in humans is encoded by the SH3GL3 gene. Enables identical protein binding activity. Predicted to be involved in synaptic vesicle uncoating. Predicted to be located in acrosomal vesicle, early endosome membrane, and presynapse. Predicted to be part of early endosome. Predicted to be active in glutamatergic synapse, postsynaptic density, intracellular component, and postsynaptic endosome.

Molecular Weight: 42 kDa

Gene ID: 6457

UniProt: Q99963