ATL1 antibody (Middle Region)

Catalog No. ABIN7603095

This Rabbit Polyclonal antibody specifically detects ATL1 in WB, IHC and FACS. It exhibits reactivity toward Human.

Quick Overview for ATL1 antibody (Middle Region) (ABIN7603095)

Target: ATL1 (Atlastin GTPase 1 (ATL1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-ATL1 Antibody

Binding Specificity: Middle Region

Purpose: Anti-SPG3A/ATL1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SPG3A/ATL1 Antibody Picoband® (ABIN7603095). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human SPG3A/ATL1, which shares 93.8% and 100% amino acid (aa) sequence identity with mouse and rat ATL1, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Abel, A., Fonknechten, N., Hofer, A., Durr, A., Cruaud, C., Voit, T., Weissenbach, J., Brice, A., Klimpe, S., Auburger, G., Hazan, J. Early onset autosomal dominant spastic paraplegia caused by novel mutations in SPG3A. Neurogenetics 5: 239-243, 2004. 2. Beetz, C., Nygren, A. O. H., Deufel, T., Reid, E. An SPG3A whole gene deletion neither co-segregates with disease nor modifies phenotype in a hereditary spastic paraplegia family with a pathogenic SPG4 mutation. (Letter) Neurogenetics 8: 317-318, 2007. 3. D'Amico, A., Tessa, A., Sabino, A., Bertini, E., Santorelli, F. M., Servidei, S. Incomplete penetrance in an SPG3A-linked family with a new mutation in the atlastin gene. Neurology 62: 2138-2139, 2004.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for ATL1

Target: ATL1 (Atlastin GTPase 1 (ATL1))

Alternative Name: ATL1

Background:

Synonyms: Eukaryotic translation initiation factor 2-alpha kinase 3, PRKR-like endoplasmic reticulum kinase, Pancreatic eIF2-alpha kinase, HsPEK, EIF2AK3, PEK, PERK

Tissue Specificity: Ubiquitous. A high level expression is seen in secretory tissues.

Background: Atlastin, or Atlastin-1, is a protein that in humans is encoded by the ATL1 gene. The protein encoded by this gene is a GTPase and a Golgi body transmembrane protein. The encoded protein can form a homotetramer and has been shown to interact with spastin and with mitogen-activated protein kinase kinase kinase kinase 4. This protein may be involved in axonal maintenance as evidenced by the fact that defects in this gene are a cause of spastic paraplegia type 3. Three transcript variants encoding two different isoforms have been found for this gene.

Molecular Weight: 64 kDa

Gene ID: 51062