CXCR2 antibody (N-Term)

Catalog No. ABIN7603138

The Rabbit Polyclonal anti-CXCR2 antibody has been validated for IHC and FACS. It is suitable to detect CXCR2 in samples from Human.

Quick Overview for CXCR2 antibody (N-Term) (ABIN7603138)

Target: CXCR2 (Chemokine (C-X-C Motif) Receptor 2 (CXCR2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCR2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-CXCR2 Antibody

Binding Specificity: N-Term

Purpose: Anti-CXCR2 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-CXCR2 Antibody Picoband® (ABIN7603138). Tested in Flow Cytometry, IHC applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human CXCR2, which shares 52.4% amino acid (aa) sequence identity with rat CXCR2.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Murphy P.M., Tiffany H.L.,"Cloning of complementary DNA encoding a functional human interleukin-8 receptor.", Science 253:1280-1283(1991). 2. Cerretti D.P., Kozlosky C.J., Vanden Bos T., Nelson N., Gearing D.P., Beckmann M.P. "Molecular characterization of receptors for human interleukin-8, GRO/melanoma growth-stimulatory activity and neutrophil activating peptide-2.", Mol. Immunol. 30:359-367(1993). 3. Sprenger H., Lloyd A.R., Lautens L.L., Bonner T.I., Kelvin D.J.,"Structure, genomic organization, and expression of the human interleukin-8 receptor B gene.",J. Biol. Chem. 269:11065-11072(1994).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CXCR2

Target: CXCR2 (Chemokine (C-X-C Motif) Receptor 2 (CXCR2))

Alternative Name: CXCR2

Background:

Synonyms: Neutrophil gelatinase-associated lipocalin,NGAL,Lipocalin-2,SV-40-induced 24P3 protein,Siderocalin LCN2,p25,Lcn2,

Tissue Specificity: Detected in lung, spleen, uterus, vagina and epididymis. .

Background: CXCR2 is a receptor for Interleukin 8, which is a powerful neutrophil chemotactic factor. It is a member of the GPCR family (subfamily, chemokine). Binding of IL8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activate a phosphatidylinositol-calcium second messenger system. This receptor binds to IL8 with a high affinity and to GRO/MGSA and NAP2 also with a high affinity. It has been reported to be expressed in a wide variety of tissues. ESTs have been isolated from human placenta and thymus libraries.

Molecular Weight: 68 kDa

Gene ID: 3579

UniProt: P25025

Pathways: cAMP Metabolic Process