EEF2 antibody (N-Term)

Catalog No. ABIN7603142

This anti-EEF2 antibody is a Rabbit Polyclonal antibody detecting EEF2 in WB, IHC, IF, ICC and FACS. Suitable for Human, Mouse, Rat and Monkey.

Quick Overview for EEF2 antibody (N-Term) (ABIN7603142)

Target: EEF2 (Eukaryotic Translation Elongation Factor 2 (EEF2))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EEF2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-EEF2 Antibody

Binding Specificity: N-Term

Purpose: Anti-EEF2/Elongation factor 2 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-EEF2/Elongation factor 2 Antibody (ABIN7603142). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human EEF2/Elongation factor 2, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat, Monkey
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for EEF2

Target: EEF2 (Eukaryotic Translation Elongation Factor 2 (EEF2))

Alternative Name: EEF2

Background:

Synonyms: Protein argonaute-1,Argonaute1,hAgo1,Argonaute RISC catalytic component 1,Eukaryotic translation initiation factor 2C 1,eIF-2C 1,eIF2C 1,Putative RNA-binding protein Q99,AGO1,EIF2C1,

Tissue Specificity: Detected in blood plasma (at protein level).

Background: Eukaryotic elongation factor 2 is a protein that in humans is encoded by the EEF2 gene. This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation.

Molecular Weight: 95 kDa

Gene ID: 1938

UniProt: P13639

Pathways: AMPK Signaling