PKM antibody (N-Term)

Catalog No. ABIN7603151

The Mouse Monoclonal anti-PKM antibody (Clone 11I4C3) (ABIN7603151) specifically detects PKM in WB, IHC, IF, FACS and ICC. The antibody is reactive with Human samples.

Quick Overview for PKM antibody (N-Term) (ABIN7603151)

Target: PKM (Pyruvate Kinase M1/2 (PKM))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PKM antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: 11I4C3

Product Details anti-PKM Antibody

Binding Specificity: N-Term

Purpose: Anti-PKM2 Antibody (monoclonal, 11I4C3)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PKM2 Antibody (monoclonal, 11I4C3) (ABIN7603151). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human PKM2, different from the related mouse sequence by five amino acids, and from the related rat sequence by four amino acids.

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for PKM

Target: PKM (Pyruvate Kinase M1/2 (PKM))

Alternative Name: PKM

Background:

Synonyms: Bcl-2 homologous antagonist/killer, Apoptosis regulator BAK, Bcl-2-like protein 7, Bcl2-L-7, BAK1, BAK, BCL2L7, CDN1

Tissue Specificity: Expressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle.

Background: PKM (Pyruvate Kinase, Muscle), also known as PK3 or PKM2, is an enzyme that in humans is encoded by the PKM gene. The activity of pyruvate kinase subtype M2 is increased by fructose 1, 6-bisphosphate (Fru-1, 6-P2). By in situ hybridization, Popescu and Cheng (1990) mapped the THBP1 gene to 15q24-q25. Ashizawa et al. (1991) manipulated the intracellular Fru-1, 6-P2 concentration in several mammalian cell lines, including human, by varying the glucose concentration in the media. Using a novel proteomic screen for phosphotyrosine-binding proteins, Christofk et al. (2008) observed that PKM2 binds ly and selectively to tyrosine-phosphorylated peptides.

Molecular Weight: 60 kDa

Gene ID: 5315

UniProt: P14618

Pathways: Warburg Effect