PKM antibody (Internal Region)

Catalog No. ABIN6264260

Product Details anti-PKM Antibody

Target: PKM (Pyruvate Kinase M1/2 (PKM))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKM antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: PKM2 Antibody detects endogenous levels of total PKM2.

Predicted Reactivity: Pig,Bovine,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human PKM2, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

References for anti-PKM antibody (ABIN6264260)

Xie, Huang, Tang, Ye, Yang, Guo, Tian, Xie, Peng, Xie: "Diallyl Disulfide Inhibits Breast Cancer Stem Cell Progression and Glucose Metabolism by Targeting CD44/PKM2/AMPK Signaling." in: Current cancer drug targets, Vol. 18, Issue 6, pp. 592-599, (2018) (PubMed).

Target Details for PKM

Target: PKM (Pyruvate Kinase M1/2 (PKM))

Alternative Name: PKM (PKM Products)

Synonyms: CTHBP antibody, OIP3 antibody, PK3 antibody, PKM2 antibody, TCB antibody, THBP1 antibody, AA414905 antibody, AL024370 antibody, AL024424 antibody, Pk-2 antibody, Pk-3 antibody, Pk3 antibody, Pkm2 antibody, PKM12 antibody, CG7070 antibody, Dmel\\CG7070 antibody, PYK antibody, Pyk antibody, cb1029 antibody, fj33f03 antibody, pkm2 antibody, pkm2a antibody, wu:fj33f03 antibody, MGC53388 antibody, PKM antibody, PK-S antibody, pk3 antibody, pkm2b antibody, zgc:92037 antibody, pyruvate kinase M1/2 antibody, pyruvate kinase, muscle antibody, Pyruvate kinase antibody, pyruvate kinase M1/2a antibody, pyruvate kinase PKM antibody, pyruvate kinase (PK) antibody, pyruvate kinase antibody, pyruvate kinase M1/2 S homeolog antibody, pyruvate kinase M1/2b antibody, PKM antibody, Pkm antibody, PyK antibody, pkma antibody, pkm antibody, LOC710798 antibody, pyk antibody, pk antibody, LOC100534543 antibody, pkm.S antibody, pkmb antibody

Background:

Description: Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.

Gene: PKM

Molecular Weight: 58 kDa

Gene ID: 5315

UniProt: P14618

Pathways: Warburg Effect