SI antibody (N-Term)

Catalog No. ABIN7603154

This anti-SI antibody is a Rabbit Polyclonal antibody detecting SI in WB, FACS and IHC. Suitable for Human, Mouse and Rat.

Quick Overview for SI antibody (N-Term) (ABIN7603154)

Target: SI

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SI antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC)

Product Details anti-SI Antibody

Binding Specificity: N-Term

Purpose: Anti-SI Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SI Antibody Picoband® (ABIN7603154). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human SI, which shares 86.1% amino acid (aa) sequence identity with rat SI.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells
1. Birkenmeier, E. H., Rowe, L. B., Leeper, L. L., Henning, S. J. The sucrase-isomaltase structural gene (Si-s) and a regulatory gene (Si-r) are closely linked to esterase-26 (Es-26) on mouse chromosome 3. Mammalian Genome 4: 531-536, 1993. 2. Chantret, I., Lacasa, M., Chevalier, G., Ruf, J., Islam, I., Mantei, N., Edwards, Y., Swallow, D., Rousset, M. Sequence of the complete cDNA and the 5-prime structure of the human sucrase-isomaltase gene: possible homology with a yeast glucoamylase. Biochem. J. 285: 915-923, 1992. 3. Davis, M. B., Green, F. R., West, L. F., Kearney, A., Povey, S., Swallow, D. M. Mapping of the gene encoding human sucrase-isomaltase (SI) to chromosome 3q25-26. (Abstract) Cytogenet. Cell Genet. 46: 604, 1987.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for SI

Target: SI

Background:

Synonyms: Sucrase-isomaltase, intestinal, Sucrase, Isomaltase, SI

Tissue Specificity: Expressed in the poorly differentiated crypt cells of the small intestine as well as in the mature villous cells. Expressed at very low levels in the colon.

Background: This gene is mapped to 3q26.1. It encodes a sucrase-isomaltase enzyme that is expressed in the intestinal brush border. The encoded protein is synthesized as a precursor protein that is cleaved by pancreatic proteases into two enzymatic subunits sucrase and isomaltase. These two subunits heterodimerize to form the sucrose-isomaltase complex. This complex is essential for the digestion of dietary carbohydrates including starch, sucrose and isomaltose. Mutations in this gene are the cause of congenital sucrase-isomaltase deficiency.

Molecular Weight: 209 kDa

Gene ID: 6476

UniProt: P14410