GABARAP antibody (N-Term)

Catalog No. ABIN7603159

The Rabbit Polyclonal anti-GABARAP antibody has been validated for WB, IHC and FACS. It is suitable to detect GABARAP in samples from Human, Mouse and Rat.

Quick Overview for GABARAP antibody (N-Term) (ABIN7603159)

Target: GABARAP (GABA(A) Receptor-Associated Protein (GABARAP))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GABARAP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-GABARAP Antibody

Binding Specificity: N-Term

Purpose: Anti-GABARAP Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-GABARAP Antibody Picoband® (ABIN7603159). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human GABARAP, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human, Mouse
1. Behrends, C., Sowa, M. E., Gygi, S. P., Harper, J. W. Network organization of the human autophagy system. Nature 466: 68-76, 2010 2. Hemelaar, J., Lelyveld, V. S., Kessler, B. M., Ploegh, H. L. A single protease, Apg4B, is specific for the autophagy-related ubiquitin-like proteins GATE-16, MAP1-LC3, GABARAP, and Apg8L. J. Biol. Chem. 278: 51841-51850, 2003. 3. Komoike, Y., Shimojima, K., Liang, J.-S., Fujii, H., Maegaki, Y., Osawa, M., Fujii, S., Higashinakagawa, T., Yamamoto, T. A functional analysis of GABARAP on 17p13.1 by knockdown zebrafish. J. Hum. Genet. 55: 155-162, 2010.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.005 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for GABARAP

Target: GABARAP (GABA(A) Receptor-Associated Protein (GABARAP))

Alternative Name: GABARAP

Background:

Synonyms: C-C chemokine receptor type 1,C-C CKR-1,CC-CKR-1,CCR-1,CCR1,Macrophage inflammatory protein 1-alpha receptor,MIP-1alpha-R,RANTES-R,CD191,Ccr1,Cmkbr1,

Tissue Specificity: Detected in the heart, spleen, lung, peritoneal exudate cells and leukocytes.

Background: Gamma-aminobutyric acid receptor-associated protein is a protein that in humans is encoded by the GABARAP gene. Gamma-aminobutyric acid A receptors [GABA (A) receptors] are ligand-gated chloride channels that mediate inhibitory neurotransmission. This gene encodes GABA (A) receptor-associated protein, which is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton.

Molecular Weight: 18 kDa

Gene ID: 11337

UniProt: O95166

Pathways: Autophagy