MAP4K1 antibody (N-Term)

Catalog No. ABIN7603180

The Rabbit Polyclonal anti-MAP4K1 antibody has been validated for WB. It is suitable to detect MAP4K1 in samples from Rat.

Quick Overview for MAP4K1 antibody (N-Term) (ABIN7603180)

Target: MAP4K1 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1 (MAP4K1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP4K1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MAP4K1 Antibody

Binding Specificity: N-Term

Purpose: Anti-MAP4K1/HPK1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-MAP4K1/HPK1 Antibody Picoband® (ABIN7603180). Tested in WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of rat MAP4K1/HPK1, which shares 88.2% and 100% amino acid (aa) sequence identity with human and mouse MAP4K1/HPK1, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Rat
1. Hu, M. C.-T., Qiu, W. R., Wang, X., Meyer, C. F., Tan, T.-H. Human HPK1, a novel human hematopoietic progenitor kinase that activates the JNK/SAPK kinase cascade. Genes Dev. 10: 2251-2264, 1996. 2. Kiefer, F., Tibbles, L. A., Anafi, M., Janssen, A., Zanke, B. W., Lassam, N., Pawson, T., Woodgett, J. R., Iscove, N. N. HPK1, a hematopoietic protein kinase activating the SAPK/JNK pathway. EMBO J. 15: 7013-7025, 1996.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for MAP4K1

Target: MAP4K1 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1 (MAP4K1))

Alternative Name: Map4k1

Background:

Synonyms: Fascin-2, Retinal fascin, FSCN2

Tissue Specificity: Localized specifically in the outer and inner segments of the photoreceptor cells in the retina.

Background: Mitogen-activated protein kinase kinase kinase kinase 1 is a protein kinase that in humans is encoded by the MAP4K1 gene. It is also known as HPK1 (Hematopoietic Progenitor Kinase 1). The protein has been shown to play a role in JNK activation.

Molecular Weight: 97 kDa

Gene ID: 292763

UniProt: D3Z8I4

Pathways: TCR Signaling, Signaling of Hepatocyte Growth Factor Receptor