MYH10 antibody (N-Term)

Catalog No. ABIN7603186

The Rabbit Polyclonal anti-MYH10 antibody has been validated for WB, IHC, ICC, IF and FACS. It is suitable to detect MYH10 in samples from Human, Mouse and Rat.

Quick Overview for MYH10 antibody (N-Term) (ABIN7603186)

Target: MYH10 (Myosin, Heavy Polypeptide 10, Non-Muscle (MYH10))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MYH10 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-MYH10 Antibody

Binding Specificity: N-Term

Purpose: Anti-non-muscle Myosin IIB/MYH10 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-non-muscle Myosin IIB/MYH10 Antibody Picoband® (ABIN7603186). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human non-muscle Myosin IIB/MYH10, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat
Immunocytochemistry/Immunofluorescence, 4 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human, Mouse, Rat
1. Ma, X., Adelstein, R. S. A point mutation in Myh10 causes major defects in heart development and body wall closure. Circ. Cardiovasc. Genet. 7: 257-265, 2014. Note: Erratum: Circ. Cardiovasc. Genet. 7: 570 only, 2014. 2. Tuzovic, L., Yu, L., Zeng, W., Li, X., Lu, H., Lu, H.-M., Gonzalez, K., Chung, W. K. A human de novo mutation in MYH10 phenocopies the loss of function mutation in mice. Rare Dis. 1: e26144, 2013. Note: Electronic Article.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for MYH10

Target: MYH10 (Myosin, Heavy Polypeptide 10, Non-Muscle (MYH10))

Alternative Name: MYH10

Background:

Synonyms: T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, Cd3e

Tissue Specificity: Highly expressed in epithelial tissues, particularly those of the gastrointestinal and respiratory tracts, such as large intestine and trachea, followed by kidney, small intestine, appendix and stomach.

Background: This gene encodes a member of the myosin superfamily. The protein represents a conventional non-muscle myosin, it should not be confused with the unconventional myosin-10 (MYO10). Myosins are actin-dependent motor proteins with diverse functions including regulation of cytokinesis, cell motility, and cell polarity. Mutations in this gene have been associated with May-Hegglin anomaly and developmental defects in brain and heart. Multiple transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 229 kDa

Gene ID: 4628

UniProt: P35580