NAPA antibody (N-Term)

Catalog No. ABIN7603194

This Rabbit Polyclonal antibody specifically detects NAPA in WB and FACS. It exhibits reactivity toward Human.

Quick Overview for NAPA antibody (N-Term) (ABIN7603194)

Target: NAPA (N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NAPA antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-NAPA Antibody

Binding Specificity: N-Term

Purpose: Anti-Alpha SNAP/NAPA Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Alpha SNAP/NAPA Antibody Picoband® (ABIN7603194). Tested in WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Alpha SNAP/NAPA, which shares 95.7% amino acid (aa) sequence identity with mouse and rat NAPA.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Andreeva, A. V., Kutuzov, M. A., Vaiskunaite, R., Profirovic, J., Meigs, T. E., Predescu, S., Malik, A. B., Voyno-Yasenetskaya, T. G-alpha-12 interaction with alpha-SNAP induces VE-cadherin localization at endothelial junctions and regulates barrier function. J. Biol. Chem. 280: 30376-30383, 2005. 2. Bronson, R. T., Lane, P. W. Hydrocephalus with hop gait (hyh): a new mutation on chromosome 7 in the mouse. Dev. Brain Res. 54: 131-136, 1990. 3. Chae, T. H., Allen, K. M., Davisson, M. T., Sweet, H. O., Walsh, C. A. Mapping of the mouse hyh gene to a YAC/BAC contig on proximal chromosome 7. Mammalian Genome 13: 239-244, 2002.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for NAPA

Target: NAPA (N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA))

Alternative Name: NAPA

Background:

Synonyms: Sodium- and chloride-dependent GABA transporter 1,GAT-1,Solute carrier family 6 member 1,SLC6A1,GABATR, GABT1, GAT1,

Tissue Specificity: Expressed in many tissues, highest levels in skeletal muscle.

Background: N-ethylmaleimide-sensitive factor Attachment Protein Alpha, also known as SNAP-α, is a SNAP protein that is involved in the intra-cellular trafficking and fusing of vesicles to target membranes in cells. This gene encodes a member of the soluble NSF attachment protein (SNAP) family. SNAP proteins play a critical role in the docking and fusion of vesicles to target membranes as part of the 20S NSF-SNAP-SNARE complex. The encoded protein plays a role in the completion of membrane fusion by mediating the interaction of N-ethylmaleimide-sensitive factor (NSF) with the vesicle-associated and membrane-associated SNAP receptor (SNARE) complex, and stimulating the ATPase activity of NSF. Alternatively spliced transcript variants have been observed for this gene.

Molecular Weight: 33 kDa

Gene ID: 8775

UniProt: P54920

Pathways: Synaptic Vesicle Exocytosis, Asymmetric Protein Localization