NUCKS1 antibody (N-Term)

Catalog No. ABIN7603231

The Rabbit Polyclonal anti-NUCKS1 antibody has been validated for WB, IHC, FACS and IF. It is suitable to detect NUCKS1 in samples from Human, Mouse and Rat.

Quick Overview for NUCKS1 antibody (N-Term) (ABIN7603231)

Target: NUCKS1 (Nuclear Casein Kinase and Cyclin-Dependent Kinase Substrate 1 (NUCKS1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NUCKS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-NUCKS1 Antibody

Binding Specificity: N-Term

Purpose: Anti-NUCKS1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-NUCKS1 Antibody Picoband® (ABIN7603231). Tested in IF, IHC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human NUCKS1, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Human, Mouse
Immunofluorescence, 5 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Grundt, K., Haga, I. V., Aleporou-Marinou, V., Drosos, Y., Wanvik, B., Ostvold, A. C. Characterisation of the NUCKS gene on human chromosome 1q32.1 and the presence of a homologous gene in different species. Biochem. Biophys. Res. Commun. 323: 796-801, 2004. 2. Ostvold, A. C., Norum, J. H., Mathiesen, S., Wanvik, B., Sefland, I., Grundt, K. Molecular cloning of a mammalian nuclear phosphoprotein NUCKS, which serves as a substrate for Cdk1 in vivo. Europ. J. Biochem. 268: 2430-2440, 2001.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for NUCKS1

Target: NUCKS1 (Nuclear Casein Kinase and Cyclin-Dependent Kinase Substrate 1 (NUCKS1))

Alternative Name: NUCKS1

Background:

Synonyms: Fms-related tyrosine kinase 3 ligand ,Flt3lg ,

Background: Nuclear ubiquitous casein and cyclin-dependent kinases substrate is a protein that in humans is encoded by the NUCKS1 gene. This gene encodes a nuclear protein that is highly conserved in vertebrates. The conserved regions of the protein contain several consensus phosphorylation sites for casein kinase II and cyclin-dependent kinases, two putative nuclear localization signals, and a basic DNA-binding domain. It is phosphorylated in vivo by Cdk1 during mitosis of the cell cycle.

Molecular Weight: 20 kDa

Gene ID: 64710

UniProt: Q9H1E3