SPOP-B antibody (N-Term)

Catalog No. ABIN7603237

The Rabbit Polyclonal anti-SPOP-B antibody has been validated for WB. It is suitable to detect SPOP-B in samples from Human.

Quick Overview for SPOP-B antibody (N-Term) (ABIN7603237)

Target: SPOP-B (Speckle-Type POZ Protein (SPOP-B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPOP-B antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SPOP-B Antibody

Binding Specificity: N-Term

Purpose: Anti-SPOP Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SPOP Antibody (ABIN7603237). Tested in WB applications. This antibody reacts with Human. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human SPOP, identical to the related mouse sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for SPOP-B

Target: SPOP-B (Speckle-Type POZ Protein (SPOP-B))

Alternative Name: SPOP

Background:

Synonyms: Tripartite motif-containing protein 6, RING finger protein 89, RING-type E3 ubiquitin transferase TRIM6, TRIM6, RNF89

Tissue Specificity: Isoform 2 is only expressed in skeletal muscle. Isoform 1 is expressed in skeletal muscle, heart, and in lesser extent in liver or pancreas. .

Background: Speckle-type POZ protein is a protein that in humans is encoded by the SPOP gene. This gene encodes a protein that may modulate the transcriptional repression activities of death-associated protein 6 (DAXX), which interacts with histone deacetylase, core histones, and other histone-associated proteins. In mouse, the encoded protein binds to the putative leucine zipper domain of macroH2A1.2, a variant H2A histone that is enriched on inactivated X chromosomes. The BTB/POZ domain of this protein has been shown in other proteins to mediate transcriptional repression and to interact with components of histone deacetylase co-repressor complexes. Alternative splicing of this gene results in multiple transcript variants encoding the same protein.

Molecular Weight: 50 kDa

Gene ID: 8405

UniProt: O43791