TNNI1 antibody (N-Term)

Catalog No. ABIN7603242

This Rabbit Polyclonal antibody specifically detects TNNI1 in WB and IHC. It exhibits reactivity toward Human.

Quick Overview for TNNI1 antibody (N-Term) (ABIN7603242)

Target: TNNI1 (Slow Skeletal Troponin I (TNNI1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNNI1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-TNNI1 Antibody

Binding Specificity: N-Term

Purpose: Anti-TNNI1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-TNNI1 Antibody Picoband® (ABIN7603242). Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human TNNI1, which shares 94.7% amino acid (aa) sequence identity with mouse and rat TNNI1.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
1. Guenet, J.-L., Simon-Chazottes, D., Gravel, M., Hastings, K. E. M., Schiaffino, S. Cardiac and skeletal muscle troponin I isoforms are encoded by a dispersed gene family on mouse chromosomes 1 and 7. Mammalian Genome 7: 13-15, 1996. 2. Tiso, N., Rampoldi, L., Pallavicini, A., Zimbello, R., Pandolfo, D., Valle, G., Lanfranchi, G., Danieli, G. A. Fine mapping of five human skeletal muscle genes: alpha-tropomyosin, beta-tropomyosin, troponin-I slow-twitch, troponin-I fast-twitch, and troponin-C fast. Biochem. Biophys. Res. Commun. 230: 347-350, 1997. 3. Wade, R., Eddy, R., Shows, T. B., Kedes, L. cDNA sequence, tissue-specific expression, and chromosomal mapping of the human slow-twitch skeletal muscle isoform of troponin I. Genomics 7: 346-357, 1990.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for TNNI1

Target: TNNI1 (Slow Skeletal Troponin I (TNNI1))

Alternative Name: TNNI1

Background:

Synonyms: Ribosome-binding protein 1, 180 kDa ribosome receptor homolog, RRp, ES/130-related protein, Ribosome receptor protein, RRBP1, KIAA1398

Tissue Specificity: Expressed in kidney.

Background: Troponin I, slow skeletal muscle is a protein that in humans is encoded by the TNNI1 gene. Troponin proteins associate with tropomyosin and regulate the calcium sensitivity of the myofibril contractile apparatus of striated muscles. Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit, blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. The TnI-fast and TnI-slow genes are expressed in fast-twitch and slow-twitch skeletal muscle fibers, respectively, while the TnI-cardiac gene is expressed exclusively in cardiac muscle tissue. This gene encodes the Troponin-I-skeletal-slow-twitch protein. This gene is expressed in cardiac and skeletal muscle during early development but is restricted to slow-twitch skeletal muscle fibers in adults. The encoded protein prevents muscle contraction by inhibiting calcium-mediated conformational changes in actin-myosin complexes.

Molecular Weight: 22 kDa

Gene ID: 7135

UniProt: P19237