TRIM25 antibody (N-Term)

Catalog No. ABIN7603254

This Rabbit Monoclonal antibody specifically detects TRIM25 in WB, IHC, IF, IP, ICC and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for TRIM25 antibody (N-Term) (ABIN7603254)

Target: TRIM25 (Tripartite Motif Containing 25 (TRIM25))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This TRIM25 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: ADFG-20

Product Details anti-TRIM25 Antibody

Binding Specificity: N-Term

Purpose: Anti-TRIM25 Monoclonal Antibody

Characteristics: Anti-TRIM25 Monoclonal Antibody (ABIN7603254). Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human TRIM25 Functions as an ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:100-1:500
IP 1:50
FC 1:60

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Concentration: Lot specific

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for TRIM25

Target: TRIM25 (Tripartite Motif Containing 25 (TRIM25))

Alternative Name: TRIM25

Background:

Synonyms: Peptidyl-prolyl cis-trans isomerase B,PPIase B,5.2.1.8,CYP-S1,Cyclophilin B,Rotamase B,S-cyclophilin,SCYLP,PPIB,CYPB,

Tissue Specificity: Expressed in brain in the thalamus, in fetal and adult lung and in placenta. .

Molecular Weight: 71 kDa

UniProt: Q14258