EIF4E antibody (pSer209)

Catalog No. ABIN7603287

This Rabbit Monoclonal antibody specifically detects EIF4E in WB, IHC, FACS and IP. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for EIF4E antibody (pSer209) (ABIN7603287)

Target: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This EIF4E antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: AFHI-5

Product Details anti-EIF4E Antibody

Binding Specificity: pSer209

Purpose: Anti-EIF4E Monoclonal Antibody

Characteristics: Anti-EIF4E Monoclonal Antibody (ABIN7603287). Tested in WB, IHC, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human EIF4E eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-5000
IHC 1:50-200
IP 1:30
FC 1:40

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Concentration: 0.5 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for EIF4E

Target: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Alternative Name: EIF4E

Background:

Synonyms: Transcriptional repressor CTCF,11-zinc finger protein,CCCTC-binding factor,CTCFL paralog,CTCF,

Tissue Specificity: Ubiquitous. Absent in primary spermatocytes. .

Molecular Weight: 25 kDa

UniProt: P06730

Pathways: BCR Signaling