SMC1A antibody (pSer957)

Catalog No. ABIN7603375

The Rabbit Monoclonal anti-SMC1A antibody has been validated for WB, IHC, IF and ICC. It is suitable to detect SMC1A in samples from Human.

Quick Overview for SMC1A antibody (pSer957) (ABIN7603375)

Target: SMC1A (Structural Maintenance of Chromosomes 1A (SMC1A))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This SMC1A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: FCI-19

Product Details anti-SMC1A Antibody

Binding Specificity: pSer957

Purpose: Anti-Phospho-SMC1 (S957) Monoclonal Antibody

Characteristics: Anti-Phospho-SMC1 Monoclonal Antibody (ABIN7603375). Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human Phospho-SMC1 (S957) Structural maintenance of chromosomes 1 (SMC1) protein is a chromosomal protein member of the cohesin complex that enables sister chromatid cohesion and plays a role in DNA repair . ATM/NBS1-dependent phosphorylation of SMC1 occurs at Ser957 and Ser966 in response to ionizing radiation (IR) as part of the intra-S-phase DNA damage checkpoint. SMC1 phosphorylation is ATM-independent in cells subjected to other forms of DNA damage, including UV light and hydroxyurea treatment.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for SMC1A

Target: SMC1A (Structural Maintenance of Chromosomes 1A (SMC1A))

Alternative Name: SMC1A

Background:

Synonyms: Red fluorescent protein eqFP611,GFP-like chromoprotein,

Tissue Specificity: Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC). Levels rapidly diminish as soon as ESC's differentiate (at protein levels). Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma. .

Molecular Weight: 15 kDa

UniProt: Q14683

Pathways: Stem Cell Maintenance