Caspase 3 antibody

Catalog No. ABIN7604230

The Rabbit Monoclonal anti-Caspase 3 antibody has been validated for WB, IHC, IP, ICC, IF and FACS. It is suitable to detect Caspase 3 in samples from Human.

Quick Overview for Caspase 3 antibody (ABIN7604230)

Target: Caspase 3 (CASP3)

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Caspase 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: AOE-3

Product Details anti-Caspase 3 Antibody

Purpose: Anti-Caspase-3 CASP3 Rabbit Monoclonal Antibody

Characteristics: Anti-Caspase-3 CASP3 Rabbit Monoclonal Antibody (ABIN7604230). Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human Caspase-3

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Concentration: Lot specific

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for Caspase 3

Target: Caspase 3 (CASP3)

Alternative Name: CASP3

Background:

Synonyms: Caspase-3,CASP-3,3.4.22.56,Apopain,Cysteine protease CPP32,CPP-32,Protein Yama,SREBP cleavage activity 1,SCA-1,Caspase-3 subunit p17,Caspase-3 subunit p12,CASP3,CPP32,

Tissue Specificity: Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.

Molecular Weight: 32 kDa

UniProt: P42574

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation