Cullin 1 antibody

Catalog No. ABIN7604533

The Rabbit Monoclonal anti-Cullin 1 antibody has been validated for WB, IHC, IF, IP, ICC and FACS. It is suitable to detect Cullin 1 in samples from Human, Mouse and Rat.

Quick Overview for Cullin 1 antibody (ABIN7604533)

Target: Cullin 1 (CUL1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Cullin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: ADIA-3

Product Details anti-Cullin 1 Antibody

Purpose: Anti-Cullin 1 Monoclonal Antibody

Characteristics: Anti-Cullin 1 Monoclonal Antibody (ABIN7604533). Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human Cullin 1 Core component of multiple cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:40
FC 1:30

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Concentration: Lot specific

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for Cullin 1

Target: Cullin 1 (CUL1)

Alternative Name: CUL1

Background:

Synonyms: Matrix metalloproteinase-14,MMP-14,3.4.24.80,MMP-X1,Membrane-type matrix metalloproteinase 1,MT-MMP 1,MTMMP1,Membrane-type-1 matrix metalloproteinase,MT1-MMP,MT1MMP,MMP14,

Tissue Specificity: Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors. .

Molecular Weight: 44 kDa

UniProt: Q13616

Pathways: Cell Division Cycle, Hedgehog Signaling, Mitotic G1-G1/S Phases, Regulation of Cell Size