Cullin 3 antibody

Catalog No. ABIN7604535

This Rabbit Monoclonal antibody specifically detects Cullin 3 in WB, IP and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Cullin 3 antibody (ABIN7604535)

Target: Cullin 3 (CUL3)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Cullin 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS)

Clone: AEOD-3

Product Details anti-Cullin 3 Antibody

Purpose: Anti-Cullin 3 Monoclonal Antibody

Characteristics: Anti-Cullin 3 Monoclonal Antibody (ABIN7604535). Tested in WB, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human Cullin 3 Core component of multiple cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-1:5000
IP 1:50
FC 1:60

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Concentration: Lot specific

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for Cullin 3

Target: Cullin 3 (CUL3)

Alternative Name: CUL3

Background:

Synonyms: G2/mitotic-specific cyclin-B1,CCNB1,CCNB,

Tissue Specificity: Ubiquitous. Expressed at high levels in testis.

Molecular Weight: 133 kDa

UniProt: Q13618

Pathways: M Phase