DDX58 antibody

Catalog No. ABIN7604629

The Rabbit Monoclonal anti-DDX58 antibody has been validated for WB and IP. It is suitable to detect DDX58 in samples from Human.

Quick Overview for DDX58 antibody (ABIN7604629)

Target: DDX58 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This DDX58 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Clone: ACEA-4

Product Details anti-DDX58 Antibody

Purpose: Anti-DDX58 Monoclonal Antibody

Characteristics: Anti-DDX58 Monoclonal Antibody (ABIN7604629). Tested in WB, IP applications. This antibody reacts with Human.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human DDX58 Retinoic acid inducible gene I (RIG-I) is a 925 amino acid, interferon-inducible cellular DExD/H box RNA helicase that activates type I interferon (IFN), an important effector of the innate immune system that is sensitive to these dsRNA viruses. dsRNA is normally present in very low quantities in cells, so when a virus is present, the elevated levels of dsRNA act as a sign telling RIG-I to activate the production of IFN.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000
IP 1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Concentration: Lot specific

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for DDX58

Target: DDX58 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58))

Alternative Name: DDX58

Background:

Synonyms: Histone H2AX,H2a/x,Histone H2A.X,H2AFX,H2AX,

Tissue Specificity: Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells.

Molecular Weight: 100-110 kDa

UniProt: O95786

Pathways: Activation of Innate immune Response, Hepatitis C