Endonuclease G antibody

Catalog No. ABIN7604746

The Rabbit Monoclonal anti-Endonuclease G antibody has been validated for WB. It is suitable to detect Endonuclease G in samples from Human, Rat and Mouse.

Quick Overview for Endonuclease G antibody (ABIN7604746)

Target: Endonuclease G (ENDOG)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Endonuclease G antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 18E31

Product Details anti-Endonuclease G Antibody

Purpose: Anti-Endo G Rabbit Monoclonal Antibody

Characteristics: Anti-Endo G Rabbit Monoclonal Antibody (ABIN7604746). Tested in WB application. This antibody reacts with Human, Mouse, Rat.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human Endo G

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Concentration: Lot specific

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for Endonuclease G

Target: Endonuclease G (ENDOG)

Alternative Name: ENDOG

Background:

Synonyms: Caspase-14,CASP-14,3.4.22.-,Caspase-14 subunit p17, mature form,Caspase-14 subunit p10, mature form,Caspase-14 subunit p20, intermediate form,Caspase-14 subunit p8, intermediate form,CASP14,

Tissue Specificity: Expressed in keratinocytes of adult skin suprabasal layers (from spinous layers to the stratum granulosum and stratum corneum) (at protein level). Expressed in keratinocytes of hair shaft and sebaceous glands (at protein level). In psoriatic skin only expressed at very low levels (PubMed:11175259). The p17/10 mature form is expressed in epidermis stratum corneum, the p20/p8 intermediate form in epidermis upper granular cells of the stratum granulosum (PubMed:22825846). .

Molecular Weight: 36 kDa

UniProt: Q14249

Pathways: Apoptosis