NDC80 antibody

Catalog No. ABIN7605080

The Rabbit Monoclonal anti-NDC80 antibody (Clone ACBI-14) (ABIN7605080) specifically detects NDC80 in WB and IP. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for NDC80 antibody (ABIN7605080)

Target: NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This NDC80 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Clone: ACBI-14

Product Details anti-NDC80 Antibody

Purpose: Anti-HEC1 NDC80 Monoclonal Antibody

Characteristics: Anti-HEC1 NDC80 Monoclonal Antibody (ABIN7605080). Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human HEC1 Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.

Isotype: IgG

Application Details

Application Notes: WB 1:500-2000
IP 1:20

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Concentration: 0.5 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for NDC80

Target: NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))

Alternative Name: NDC80

Background:

Synonyms: Eukaryotic translation initiation factor 5A-1,eIF-5A-1,eIF-5A1,Eukaryotic initiation factor 5A isoform 1,eIF-5A,Rev-binding factor,eIF-4D,EIF5A,

Tissue Specificity: Expressed in umbilical vein endothelial cells and several cancer cell lines (at protein level). .

Molecular Weight: 68 kDa

UniProt: O14777

Pathways: Maintenance of Protein Location