Histone H3.3 antibody

Catalog No. ABIN7605121

The Rabbit Monoclonal anti-Histone H3.3 antibody has been validated for WB, IHC, IF and ICC. It is suitable to detect Histone H3.3 in samples from Human, Mouse and Rat.

Quick Overview for Histone H3.3 antibody (ABIN7605121)

Target: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Histone H3.3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: EBF-8

Product Details anti-Histone H3.3 Antibody

Purpose: Anti-Histone H3.3 H3F3A Rabbit Monoclonal Antibody

Characteristics: Anti-Histone H3.3 H3F3A Rabbit Monoclonal Antibody (ABIN7605121). Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human Histone H3.3

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000
IHC 1:500-1:1000
ICC/IF 1:500-1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Concentration: Lot specific

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for Histone H3.3

Target: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Alternative Name: H3F3A

Background:

Synonyms: Histone H3.3,H3F3A,H3.3A, H3F3,PP781,H3F3B,H3.3B,

Tissue Specificity: Expressed in a discontinuous manner in the basal cell layer of adult skin epidermis, but continuously in the basal layer of fetal skin epidermis and nail. Also expressed in the outer root sheath above the hair bulb in hair follicle (at protein level). Expressed homogeneously in all cell layers of the esophagus and exocervix, but detected in the basal cell layer only of oral mucosa, skin and in the basal plus the next two layers of the suprabasal epithelium of the palate. .

Molecular Weight: 94 kDa

UniProt: P84243