Recombinant Histone H3.3 antibody

Catalog No. ABIN5557308

This Rabbit Monoclonal antibody specifically detects Histone H3.3 in WB, IF, IHC (p) and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for Recombinant Histone H3.3 antibody (ABIN5557308)

Target: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Histone H3.3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Histone H3.3 Antibody

Purpose: Histone H3.3 Recombinant Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: Recombinant human Histone H3.3 protein, full length.

Isotype: IgG

Application Details

Application Notes: WB(1:200-1000), IHC-P(1:100-400), IHC-F(1:100-400), IF(1:100-500)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M TBS ( pH 7.4), 1 % BSA, 0.02 % Proclin 300, and 50 % Glycerol

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for Histone H3.3

Target: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Alternative Name: Histone H3.3

Background:

Synonyms: H3F3, H3.3A, Histone H3.3, H3F3A, PP781

Background: Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Gene ID: 3020

UniProt: P84243