LIN28A antibody

Catalog No. ABIN7605429

This Rabbit Monoclonal antibody specifically detects LIN28A in WB, IHC, IF, FACS, ICC and IP. It exhibits reactivity toward Human.

Quick Overview for LIN28A antibody (ABIN7605429)

Target: LIN28A (Lin-28 Homolog A (C. Elegans) (LIN28A))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This LIN28A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Clone: AFFE-12

Product Details anti-LIN28A Antibody

Purpose: Anti-LIN28 LIN28A Monoclonal Antibody

Characteristics: Anti-LIN28 LIN28A Monoclonal Antibody (ABIN7605429). Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human LIN28 Involved in the timing of developmental events and choice of stage specific cell fates. Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7.

Isotype: IgG

Application Details

Application Notes: WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:50
FC 1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Concentration: 0.5 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for LIN28A

Target: LIN28A (Lin-28 Homolog A (C. Elegans) (LIN28A))

Alternative Name: LIN28A

Background:

Synonyms: Protein lin-28 homolog A,Lin-28A,Zinc finger CCHC domain-containing protein 1,LIN28A,CSDD1, LIN28, ZCCHC1,

Tissue Specificity: Expressed in embryonic stem cells, placenta and testis. Tends to be up-regulated in HER2-overexpressing breast tumors. .

Molecular Weight: 33 kDa

UniProt: Q9H9Z2

Pathways: Stem Cell Maintenance