LIN28B antibody

Catalog No. ABIN7605431

The Rabbit Monoclonal anti-LIN28B antibody (Clone EAA-12) (ABIN7605431) specifically detects LIN28B in WB, FACS, IF, IP and ICC. The antibody is reactive with Human samples.

Quick Overview for LIN28B antibody (ABIN7605431)

Target: LIN28B (Lin-28 Homolog B (LIN28B))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This LIN28B antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: EAA-12

Product Details anti-LIN28B Antibody

Purpose: Anti-Lin28B Rabbit Monoclonal Antibody

Characteristics: Anti-Lin28B Rabbit Monoclonal Antibody (ABIN7605431). Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human Lin28B

Isotype: IgG

Application Details

Application Notes: WB 1:500-2000
ICC/IF 1:50-200
IP 1:20
FC 1:20

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Concentration: 0.5 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for LIN28B

Target: LIN28B (Lin-28 Homolog B (LIN28B))

Alternative Name: LIN28B

Background:

Synonyms: Protein lin-28 homolog B,Lin-28B,LIN28B,CSDD2,

Tissue Specificity: Expressed at high levels in the placenta and, at mucher lower, in testis and fetal liver (PubMed:16971064). Isoform 1 is only detected in placenta and in moderately and poorly differentiated hepatocellular carcinoma cells (at protein level). Isoform 2 is detected in fetal liver, non-tumor liver tissues, as well as well-differentiated tumor tissues (at protein level). Tends to be up-regulated in triple-negative (ER-,PR-,HER2-) breast tumors, as well as in liver, ovarian, and thyroid carcinomas (PubMed:22118463). .

Molecular Weight: 35 kDa